normal plasma frozen pool  (Affinity Biologicals)

Journal: HemaSphere

Article Title: Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure

doi: 10.1097/HS9.0000000000000166

Figure Lengend Snippet: Fibrinography reproducibility. Fibrinography was performed as described in material and methods for our normal plasma pool (blue curves, fibrinogen = 2.5 g/L), heparinized plasma (green curves, fibrinogen = 2.8 g/L), and def-PS plasma (red curves, fibrinogen = 2.4 g/L). For each plasma, experiment was repeated 40 times on different days. All the curves are represented on the graph with the individual curves in grey centered by the mean curves (thick colored curves). All the individual curves are contained in the ±2 standard deviations curves (light colored curves).

Article Snippet: A frozen normal plasma pool (NP) was obtained from normal donors; its final fibrinogen concentration was 2.5 g/L (Diagnostica Stago).

Techniques: Clinical Proteomics

Journal: HemaSphere

Article Title: Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure

doi: 10.1097/HS9.0000000000000166

Figure Lengend Snippet: Effect of fibrinogen concentration of Fibrinography. The 50% diluted normal plasma pool was spiked with purified fibrinogen in order to generate fibrinogen concentrations ranging from 1.2 (diluted plasma) to 11.3 g/L. Plasma was coagulated at 37°C as described in the Materials and Methods Section with 2 pM TF, 4 μM PL, and 16.7 mM CaCl 2 . (A) Final protofibrils number as a function of fibrinogen concentration (mean ± SD). Bottom panel: Confocal microscopy images obtained for selected concentrations of fibrinogen. (B) Confocal microscopy images were analyzed according to Munster and Fabry to determine the pore sizes inside the fibrin clots (mean ± SD). A typical histogram of pore size is represented in the inset. PdF = pore size probability distribution function.

Article Snippet: A frozen normal plasma pool (NP) was obtained from normal donors; its final fibrinogen concentration was 2.5 g/L (Diagnostica Stago).

Techniques: Concentration Assay, Clinical Proteomics, Purification, Confocal Microscopy, Pore Size

Journal: HemaSphere

Article Title: Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure

doi: 10.1097/HS9.0000000000000166

Figure Lengend Snippet: Effect of fibrinogen concentration on the single wavelength optical density of the clot. The 50% diluted normal plasma pool was spiked with purified fibrinogen in order to generate fibrinogen concentrations ranging from 1.2 (diluted plasma) to 11.3 g/L. Plasma was coagulated at 37°C as described in the Materials and Methods Section with 2 pM TF, 4 μM PL, and 16.7 mM CaCl 2 . Final optical density at 540 nm was extracted from the spectra and plotted over fibrinogen concentration expressed as mean ± SD.

Article Snippet: A frozen normal plasma pool (NP) was obtained from normal donors; its final fibrinogen concentration was 2.5 g/L (Diagnostica Stago).

Techniques: Concentration Assay, Clinical Proteomics, Purification